Cau 3 : cac buoc trong ki thuat di truyen:

- B1 :  Nuoi tb chu de tao vector chuyen gen va nuoi tb chob ( nhu tb nhan ) de chuyen ADN

- B2: Tach chiet ADN plashmid tu tb VK va ADN tb cho.

-B3 : cat ca 2 loai ADN ( ADN plshmid va ADN tb cho bang cung 1 ;laoi enxyme gioi han ( restriction endonuclease - RE ) VD : dung enzyme gioi hanendonulease EcorI tao cac dau sole co biet ( cohensive ends )

- B4 : tron chung ADN plashmid da bj cat voi doan ADN tb cung bj cat boi cung 1 loai enzyme gioi han nhu da neu tren.

- B5 : them enzyme noi ( ligasa ) de tao ra ADN tai to hop hoan chjnh.

- B6 : Bien nap ADN tai to hop vao tb chu ( VD : vk Ecoli ) va nhan dong.

- B7 : chon loc va tao dong vk mang gen la.