cau 8 >

Co 3 pp :

A, THu nhan nguon gen tu he gen : Thu vien gen la tap hop cac doan ADN ngau nhien cua cung 1 loai nhat dinh ma dc nap vao 1 tap doan vector tuong ung ( plasmid, phage, cosmid ) va sau do dc dua vao ki chu thjch hop, dc nhan len thanh dong va tap hpop nay fai du lon.

UU diem : don gian, de lam , luu tru dc nhieu gen

Nhuoc diem : cat kho chjnh xac dc doan gen mong muon

b, Sinh tong hop tu m ARN tuong ung

------------------------------------ mARN

                               I                             - enzyme ien ma ngc + oligdt

---------------------------------------m ARN ( A,U,G,C )

                               I                    c ADN 9I) ( A,T,G,U )

--------------------------------------        c ADN

(_____________________

                               [      nuclease S1

-------------------------------------------- c ADN

___________________________

Uu diem : doan ADn thu dc la 1 1 doan ADN tinh dung nhu ta mong muon

Nhuoc diem : chj su dung ra 1 pp duy nhat

c, Tong hop bang pp hoa hoc

UU : Neu biet trinh tu sap xep cua doan nucleotit thi co the tong hong tat ca cac doan gen

Nhuoc : chj tong hop nhung doan gen ngan , k the tong hop dc cac doan gen wa dai.